Paul Singleton

Dictionary of DNA and Genome Technology

Автор Paul Singleton
Формат PDF
Рейтинг книги
(оценок < 5)
0 10

‘DNA technology’ refers to a variety of procedures – some simple, others extremely complex and sophisticated. At the most basic level they include: isolating and purifying DNA; cutting DNA with specialized enzymes; characterizing, sequencing and expressing DNA; hybridizing, labeling, ligating, staining and quantifying DNA; and amplifying specific regions of DNA. This technology is used in fields such as: • diagnosis (infectious and hereditary diseases) • drug development • epidemiology • forensic science • gene therapy • genetically modified (GM) foods • industrial enzymes • oncology • taxonomy • vaccine development Covering much of the established methodology, and some background material, the dictionary also contains many items of new information first reported in mainstream journals within the last 15 months – for example: new methods for amplifying DNA; DFRS plasmids; combined probe–dye monitoring of realtime PCR; binary deoxyribozyme ligase; footprinting in vivo with X-radiation; oligonucleotide–peptide conjugation by Diels–Alder chemistry; new notation for DNA; benzylguanine labels for fusion proteins; amplifying damaged DNA; chromosomal ccdB gene for plasmid maintenance; and GFP reactivation in methylation analysis.